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Fig. 1 | BMC Cancer

Fig. 1

From: Lymphocyte-specific protein tyrosine kinase (Lck) interacts with CR6-interacting factor 1 (CRIF1) in mitochondria to repress oxidative phosphorylation

Fig. 1

Mitochondrial localization of endogenous Lck protein in both mouse and human leukemia cell lines. (a) Mitochondrial (Mito) fractions isolated from three leukemia cell lines were analyzed by Lck immunoblotting. Immunoblotting for VDAC1 (mitochondrial marker), GAPDH (cytoplasmic marker), and lamin B1 (nuclear marker) was performed to verify purity of mitochondrial fractions. Jcam whole cell lysate (WCL) was used as the positive control for markers. LSTRA lysates were analyzed on a separate membrane as shown by the dotted lines. (b) Confocal microscopy of three-color fluorescence staining of Jurkat (top and middle panels) and Jcam (bottom panels) cells. An area of Jurkat microscopy (bordered with white lines) is enlarged and shown on the right. Lck (red) and mito-tracker (green) co-localization are shown as yellow dots and depicted by white arrows in the enlarged image. Nuclei are visualized with DAPI staining (blue). Scale bars are shown in the bottom

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BMC Cancer

ISSN: 1471-2407

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