Fig. 5

Lower levels of mitochondrion-encoded OXPHOS proteins and abnormal mitochondrial structure in Jurkat.( a) Normalized whole cell lysates from Jurkat and Jcam cells were analyzed by Western blot using antibodies specific for ND1, COI, COIV, VDAC1 and GAPDH. Signal intensity was quantitated for ND1 and COI and fold change is indicated below the images. (b) Total RNAs isolated from Jurkat and Jcam cells were subjected to real-time PCR using primers specific for human ND1, COI and COIV. Data from triplicates were normalized to actin and expressed as fold change of Jurkat in comparison to Jcam. Statistical analyses show no significant difference from three independent studies. (c) Transmission electron microscopy of Jurkat (lower panels) and Jcam (upper panels) cells. An area with enriched mitochondria is bordered with black lines and enlarged on the right to better visualize detailed intramitochondrial structure. Black arrows denote several representative mitochondria. The position of nucleus at the upper-left corner is also labeled as “N”. Scale bars are shown in the lower-right corners of microscopy images