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Table 1 Lymphangiogenic signaling-related and lymphatic specific markers

From: A potential small-molecule synthetic antilymphangiogenic agent norcantharidin inhibits tumor growth and lymphangiogenesis of human colonic adenocarcinomas through blocking VEGF-A,-C,-D/VEGFR-2,-3 “multi-points priming” mechanisms in vitro and in vivo

Cells

Genes

PCR primers (forward-reverse)

HCACCs and the co-culture system in vitro

VEGF-A

5′-CAC CGC CTC GGC TTG TCA CAT-3

5′-CTG CTG TCT TGG GTG CAT CTG-3′

VEGF-C

5′-ACC TGC CCC ACC AAT TAC A-3′

5′-GCC TCT TGT AAA GAC TGG TT-3′

VEGF-D

5′-GCT GTT GCA ATG AAG AGA CG-3′

5′-TCT TCT GTT CCA GCA AGT GC-3′

VEGFR-2

5′-CAC ACA GAG ATG ATT ACT ACA CTG-3′

5′-CCA TCT TGA GCA TCA GAT CC TC-3′

VEGFR-3

5′-AAG TAC ATC AAG GCA CGC ATC GAG-3′

5′-GGC TTG TTG ATG AAT GGC TGC TCA-3

GAPDH

5′-ACA GAG CCT CGC CTT TGC C-3′

5′-CAT GTC GTC CCA GTT GGT G-3′

In-situ xenograft cells in vivo

VEGF-A

5′-CTG CTC GCC GCT GCG CTG-3′

5′-GTG CTG GTG TTC ATG CAC TGC AG-3′

VEGF-C

5′-GCC ACG GCT TATG CAA GCA AAG AT-3′

5′-AGT TGA GGT TGG CCT GTT CTC TGT-3′

VEGF-D

5′-CGA TGT GGT GGC TGT TGC AAT GAA-3′

5′-GCT GTT GGC AAG CAC TTA CAA CCT-3′

VEGFR-2

5′-CGG AGT CAA CGG ATT TGG TCG TAT-3′

5′-AGC CTT CTC CAT GGT GGT GAA GAC-3′

VEGFR-3

5′-GAC AGC TAC AAG TAC GAG CAT CTG-3′

5′-CGT TCT TGC AGT CGA GCA GAA-3′

CK-20

5′-CAG ACA CAC GGT GAA CTAT GG-3′

5′-GAT CAG CTT CCA CTG TTA GAC G-3′

LYVE-1

5′-TGC AGA ATT ATG GGG ATC AC-3′

5′-GGC TGT TTC AAC TTG GTC CT-3′

D2-40

5′-GGT GCC GAA GAT GAT GTG-3′

5′-CGA TGC GAA TGC CTG TTA-3

GAPDH

5′-GCA CCA CCA ACT GCT TA-3′

5′-AGT AGA GGC AGG GAT GAT-3′