Cells | Genes | PCR primers (forward-reverse) |
---|---|---|
HCACCs and the co-culture system in vitro | VEGF-A | 5′-CAC CGC CTC GGC TTG TCA CAT-3′ |
5′-CTG CTG TCT TGG GTG CAT CTG-3′ | ||
VEGF-C | 5′-ACC TGC CCC ACC AAT TAC A-3′ | |
5′-GCC TCT TGT AAA GAC TGG TT-3′ | ||
VEGF-D | 5′-GCT GTT GCA ATG AAG AGA CG-3′ | |
5′-TCT TCT GTT CCA GCA AGT GC-3′ | ||
VEGFR-2 | 5′-CAC ACA GAG ATG ATT ACT ACA CTG-3′ | |
5′-CCA TCT TGA GCA TCA GAT CC TC-3′ | ||
VEGFR-3 | 5′-AAG TAC ATC AAG GCA CGC ATC GAG-3′ | |
5′-GGC TTG TTG ATG AAT GGC TGC TCA-3 | ||
GAPDH | 5′-ACA GAG CCT CGC CTT TGC C-3′ | |
5′-CAT GTC GTC CCA GTT GGT G-3′ | ||
In-situ xenograft cells in vivo | VEGF-A | 5′-CTG CTC GCC GCT GCG CTG-3′ |
5′-GTG CTG GTG TTC ATG CAC TGC AG-3′ | ||
VEGF-C | 5′-GCC ACG GCT TATG CAA GCA AAG AT-3′ | |
5′-AGT TGA GGT TGG CCT GTT CTC TGT-3′ | ||
VEGF-D | 5′-CGA TGT GGT GGC TGT TGC AAT GAA-3′ | |
5′-GCT GTT GGC AAG CAC TTA CAA CCT-3′ | ||
VEGFR-2 | 5′-CGG AGT CAA CGG ATT TGG TCG TAT-3′ | |
5′-AGC CTT CTC CAT GGT GGT GAA GAC-3′ | ||
VEGFR-3 | 5′-GAC AGC TAC AAG TAC GAG CAT CTG-3′ | |
5′-CGT TCT TGC AGT CGA GCA GAA-3′ | ||
CK-20 | 5′-CAG ACA CAC GGT GAA CTAT GG-3′ | |
5′-GAT CAG CTT CCA CTG TTA GAC G-3′ | ||
LYVE-1 | 5′-TGC AGA ATT ATG GGG ATC AC-3′ | |
5′-GGC TGT TTC AAC TTG GTC CT-3′ | ||
D2-40 | 5′-GGT GCC GAA GAT GAT GTG-3′ | |
5′-CGA TGC GAA TGC CTG TTA-3 | ||
GAPDH | 5′-GCA CCA CCA ACT GCT TA-3′ | |
5′-AGT AGA GGC AGG GAT GAT-3′ |