Fig. 5

Whole tumour slide imaging. HIF1 alpha: (a) in control tumours, the HIF1alpha expression is mainly located inside heaps of high cellularity (b) in treated ones, the labelling is weaker, globally as necrotic regions get larger, and even locally inside living cell islets. TUNEL: (c) control section with low level of apoptosis (d) highly apoptotic sunitinib-treated tumour. Ki-67: (e) proliferating cell density remains at a relatively low level in control tumours, whereas (f) in treated ones, necropsied areas get larger but the density of Ki-67 positive cells increases in the remaining living cell islets. F4/80: (g) in controls, the highest TAMs density is encountered at the interface of tumour and necrotic regions; (h) in treated tumours, TAMs tend to relocate at the tumour external edges. GLUT1: (i) in control conditions, necrotic areas are the place of high GLUT1 expression; (j) under sunitinib treatment, necropsied areas are larger and GLUT1 expression changed accordingly