Table 1 Primers used
OMIM | Symbol | Forward primer | Reverse primer | Annealing (°C) | Size (bp) | Accession number |
|---|---|---|---|---|---|---|
Target genes | ||||||
CTNNB1 | β-Catenin | ATGGGTAGGGCAAATCAGTAAGAGGT | AAGCATCGTATCACAGCAGGTTAC | 64.0 | 106 | XM_005634157.1 |
AXIN2 | Axin2 | GGACAAATGCGTGGATACCT | TGCTTGGAGACAATGCTGTT | 60.0 | 141 | XM_548025 |
BNIPL | BCL2 | TGGAGAGCGTCAACCGGGAGATGT | AGGTGTGCAGATGCCGGTTCAGGT | 62.0 | 87 | AY_509563.1 |
CCND1 | CYCLIND1 | GCCTCGAAGATGAAGGAGAC | CAGTTTGTTCACCAGGAGCA | 60.0 | 117 | NM_001005757.1 |
ERBB1 | EGFR | CTGGAGCATTCGGCA | TGGCTTTGGGAGACG | 53.0 | 107 | XM_533073 |
ERBB2 | HER2 | CGTGCTGGACAATGGAGACC | CCGCTGAATCAAGACCCCTC | 64.0 | 51 | AB008451 |
ERBB3 | HER3 | TAGTGGTGAAGGACAACGGCAG | GGTCTTGGTCAATGTCTGGCAG | 70.0 | 103 | XM_538226 |
HSP90AA1 | HSP90 | CTTGACCGATCCCAGTAAGC | TATTGATCAGGTCGGCCTTC | 59.0 | 127 | XR_134513.2 |
ID1 | ID1 | CTCAACGGCGAGATCAG | GAGCACGGGTTCTTCTC | 59.5 | 135 | XM_847117.2 |
ID2 | ID2 | GCTGAATAAATGGTGTTCGTG | GTTGTTCTCCTTGTGAAATGG | 60.5 | 114 | XR_134413.1 |
TCF7L3 | LEF1 | AGACATCCTCCAGCTCCTGA | GATGGATAGGGTTGCCTGAA | 60.0 | 137 | XP_863334.2 |
MET | cMET | TGTGCTGTGAAATCCCTGAATAGAATC | CCAAGAGTGAGAGTACGTTTGGATGAC | 56.0 | 159 | NM_001002963.1 |
MUC1 | MUC1 | CTATGAGGAGGTTTCTGCAG | GAACACAGTTGAGAGGAGAG | 62.0 | 172 | NM_001194977 |
NCOA3 | NCOA3 | ATGCGGCCTGGTGAGATT | TAAGAAGTGGCCTATTTTGAGTCC | 67.1 | 141 | ENSCAFT00000017243 |
PTEN | PTEN | AGATGTTAGTGACAATGAACCT | GTGATTTGTGTGTGCTGATC | 62.0 | 102 | NM_001003192.1 |
RAC1 | RAC1 | TCCCTTATCCTATCCGCAAA | ATGATAGGGGTGTTGGGACA | 58.0 | 128 | NM_001003274.2 |
RAC1B | RAC1b | TGGGATACAGCTGGACAAGA | CTTGTCTTTGCCCCTGGAG | 58.0 | 108 | JN_182651.1 |
SNAI2 | SLUG | CTTCACTCCGACTCCAAACG | TGGATTTTGTGCTCTTGCAG | 60.0 | 147 | XM_005637933.1 |
Reference genes | ||||||
HMBS | HMBS | TCACCATCGGAGCCATCT | GTTCCCACCACGCTCTTCT | 61.0 | 112 | XM_546491 |
HNRNPH2 | HNRPH | CTCACTATGATCCACCACG | TAGCCTCCATAACCTCCAC | 61.2 | 151 | XM_538576 |
HPRT1 | HPRT | AGCTTGCTGGTGAAAAGGAC | TTATAGTCAAGGGCATATCC | 57.0 | 104 | NM_001003357 |
RPS5 | RPS5 | TCACTGGTGAGAACCCCCT | CCTGATTCACACGGCGTAG | 62.5 | 141 | XM_533568 |
RPS19 | RPS19 | CCTTCCTCAAAAAGTCTGGG | GTTCTCATCGTAGGGAGCAAG | 62.0 | 95 | XM_533657 |
SRPR | SRPR | GCTTCAGGATCTGGACTGC | GTTCCCTTGGTAGCACTGG | 61.2 | 81 | XM_546411 |
TBP | TBP | CTATTTCTTGGTGTGCATGAGG | CCTCGGCATTCAGTCTTTTC | 57.0 | 96 | XM_849432 |
YWHAZ | YWHAZ | CGAAGTTGCTGCTGGTGA | TTGCATTTCCTTTTTGCTGA | 58.0 | 96 | XM_843951 |