Fig. 1

Sulindac and sulindac sulfone inhibit colon cancer cell growth without decreasing expression of Sp1, Sp3 and Sp4 proteins. a, b Sulindac and sulindac sulfone inhibit SW480 and RKO cell proliferation. Cells were treated with solvent control (DMSO), 600 or 900 μM sulindac, or 225 or 300 μM sulindac sulfone for 24, 48 and 72 h. Cell numbers were determined as described under Materials and Methods. Experiments were done in triplicate and results are expressed as mean ± S.D. for each determination. c, d Sulindac and sulindac sulfone have no effect on expression of Sp proteins in SW480 and RKO cells. Cells were treated with DMSO, 600 or 1200 μM sulindac, or 225 or 300 μM sulindac sulfone for 24 and 48 h. Sp1, Sp3 and Sp4 cellular levels were determined by Western blot analysis as described under Materials and Methods and β-actin was used as loading control