Fig. 1

Expression of NETO2 in primary CRC tissues. a NETO2 mRNA expression in 57 paired human CRC and corresponding adjacent normal mucosa specimens were determined by real-time qPCR method. Gene expression results were normalized by internal control β-actin. (T, tumor tissues; N, adjacent normal tissues). b Protein expression levels of NETO2 in an independent set of 24 paired CRC and corresponding adjacent normal specimens were determined by Western blot analysis. β-actin was used as a loading control. The relative protein expression of NETO2 was quantified and normalized to β-actin. Each N was arbitrarily designated 1.0. (T: Tumor tissues; N: adjacent normal tissues, T vs N, p < 0.001)