Fig. 7
Differential effects of αNF on BRCA-1 and ERα expression in MCF-7 and UACC-3199 breast cancer cells. Cells were cultured for 72 h in control phenol red-free media (DMEM for MCF-7; RPMI for UACC-31299) supplemented with 10 % charcoal-stripped FCS in the presence or absence of 10 nM E2, alone or in combination with 2 μM αNF. Bands are representative immunocomplexes detected by Western blotting for BRCA-1, ERα, and GAPDH from two independent experiments performed in duplicate (n = 4)