Fig. 4

Expression of CD133⁺ cells after IFN-γ treatment in vitro and in vivo. a Left, representative percentage of CD133⁺ cells in four HCC cell lines with or without 10 ng/ml IFN-γ treatment for four days analyzed by flow cytometry. Control, treated with buffer control; IFN-γ, treated with 10 ng/ml IFN-γ. Right, Q-PCR results of CD133 mRNA expression in four HCC cell lines with or without 10 ng/ml IFN-γ treatment for four days. C, treated with buffer control; I, treated with 10 ng/ml IFN-γ. **, p < 0.01. Representative of three independent experiments. b Left, representative percentage of CD133⁺ cells in the indicated cell line-implanted nude mice with or without IFN-γ treatment for four weeks analyzed by flow cytometry. Right, summary figure.*, p < 0.05. Gating strategy, all gating of live tumor cells