Fig. 1

Immunohistochemical staining of ampullary cancer. Subtype (a-d; g-j) and EMT (e&f; k&l) histomorphologoical and immunohistological analysis for the intestinal (a-f) and pancreatobiliary (g-l) AMPAC subtype, taken at 40-fold magnification. HE (a&g) staining representing the pancreaticobilliary (a) subtype with cuboidal formed columnar tumor cells and rounded nuclei, membranous KRT7 (c) positivity, CDX2 (b) and KRT20 (d) negativity and the intestinal type (g) with pseudostratified mucin producing glandular epithelium, elongated hyperchromatic and pseudostratified nuclei, nuclear CDX2 (h) and membranous KRT20 (j) positivity and KRT7 negativity (i). Nuclear ZEB1 expression (e, red arrows) is linked with a immunohistological membranous to cytoplasmatic Ecad shuttling. Contrary absent ZEB1 expression is immunohistological accompanied with membranous Ecad expression. Abbreviation: EMT Epithelial-Mesenchymal-Transition; AMPAC Ampullary Adenocarcinoma; HE Hematoxylin-Eosin, KRT Cytokeratin, CDX2 Caudal Type Homeobox 2, ZEB1 Zinc finger E-box binding homeobox 1, Ecad E-Cadherin