Fig. 6

Cell invasion and gene expression changes in ampullary cancer cell lines treated with CAF conditioned medium. Cell invasion was quantified by matrigel transmigration assay and mRNA expression by real time PCR. a baseline matrigel transmigration relative to most invasive cell line RCB1280. b effect of CAF-CM on intestinal-like cell line SNU869 and pancreatobiliary-like cell line SNU478. c effect of CAF-CM on mesenchymal-like cell lines MDA-AMP7, AVC1 and RCB1280. Diagrams depict mean and error bars standard deviation from n = 4 measurements, * p < 0.05 in two-sided t-test compared to untreated control. Abbreviations: AMP7 MDA-AMP7, CAF-CM cancer associated fibroblast conditioned medium, ZEB1 zinc finger E-box binding homeobox 1, CDH1 Cadherin 1 = E-Cadherin, KRT Cytokeratin, CDX2 Caudal type homeobox 2