Characterization of various cell lines from different ampullary cancer subtypes and cancer associated fibroblast-mediated responses

Table 5 Gene Ontology (GO) functional enrichment analysis regulated proteins in ampullary cancer cells MDA-AMP7, AVC1, RCB1280 and SNU487

GO terms of up-regulated proteins	Count	FDR
GO:0065007 Biological regulation	93	1.41E-02
GO:0071840 Cellular component organization or biogenesis	67	2.08E-06
GO:0051179 Localization	54	5.18E-04
GO:0016071 mRNA metabolic process	42	9.87E-23
GO:0032268 Regulation of cellular protein metabolic process	44	5.73E-11
GO:0050896 Response to stimulus	75	1.09E-02
GO terms of down-regulated proteins	Count	FDR
GO:0008152 Metabolic process	98	9.97E-04
GO:0009987 Cellular process	119	3.15E-03
GO:0044237 Cellular metabolic process	113	4.87E-11
GO:0009117 Nucleotide metabolic process	37	2.96E-12
GO:0016310 Phosphorylation	22	2.60E-03
GO:0006629 Lipid metabolic process	24	5.66E-04

Enrichment is analyzed using all differentially expressed proteins with fold-change of greater than 50 % in MDA-AMP7, AVC1, RCB1280 and SNU487 cells cultivated in CAF-conditioned medium versus non-treated control cells (log₂ ratio of > 0.58, p-value < 0.1, two-tailed t-test, FDR < 0.05)
Abbreviations: CAF cancer associated fibroblast, FDR false discovery rate

ISSN: 1471-2407