Table 2 Characteristics of antibodies used
Antibody | Supplier/Company | Methods of antigen retrieval | AB dilution | Conditions of incubation |
|---|---|---|---|---|
Anti-AGE | TransGenic | Microwave 20 min | 1:500 | Overnight |
(monoclonal mouse) | Citrate buffer (pH6) | 4 °C | ||
Anti-RAGE | R&D | Microwave 20 min | 1:500 | Overnight |
(polyclonal goat) | Citrate buffer (pH6) | 4 °C | ||
Anti-Glyoxalase-I | Santa Cruz | Microwave 20 min | 1:300 | Overnight |
(monoclonal mouse) | Biotechnology | Citrate buffer (pH6) | 4 °C | |
Anti-Adiponectin Receptor Type 1 | Phoenix | Microwave 20 min | 1:500 | 60 min |
(polyclonal rabbit) | Pharmaceuticals | Citrate buffer (pH6) | 4 °C | |
Anti-Adiponectin Receptor Type 2 | Phoenix | Microwave 20 min | 1:500 | Overnight |
(polyclonal rabbit) | Pharmaceuticals | Citrate buffer (pH6) | 4 °C |