Fig. 2

Progression of apoptosis is associated with reduced CA IX expression in CGL3 cells. a FACS analysis of the normoxic CGL3 cells treated with 20 μg/ml CHX and stained for CA IX and DNA content as described in Fig. 1d. Graph shows the percentage of the subG1 apoptotic cells in the CA IX-negative and the CA IX-positive subpopulations and changes in the percentage of the CA IX-positive cells. b Immunoblotting analysis of the CA IX level before (−) and after the CHX treatment (+) in whole cell population, and in the cells that died or survived the CHX treatment. c ELISA assessment of the relative CA IX levels in the CHX-treated CGL3 cells. Error bars represent the mean ± SD,*p < 0.05. d, e FACS analyses of the CGL3 cells treated with actinomycin D (ActD) and dexamethasone (DX), stained as in Fig. 1d. The data show reduced proportion of the CA IX-positive cells in the course of the treatment and faster progression of apoptosis in the CA IX-negative cells