Fig. 4
From: Epigenetic silencing of miR-181c by DNA methylation in glioblastoma cell lines
Inducible knockdown of human CTCF in U87MG glioblastoma cell line affects miR-181c expression. a Western blot shows CTCF protein levels in U87MG cells transduced with an inducible Empty Vector (EV) with and without Doxycycline (DOX) induction as controls. U87MG cells were also transduced with an inducible shRNAi against CTCF without (−DOX) and with (+DOX) Doxycycline (DOX). b primiR-181c expression levels in cell pools containing the inducible shRNAi against CTCF. No treatment (U87MG/shRNAi), treatment (U87MG/shRNAi/+DOX) and 2 weeks after Doxycycline deprivation (U87MG/shRNAi/2 weeks/post-DOX) were measured by RT-qPCR with SYBR Green. Empty vector controls are shown (U87MG/EV-DOX and U87MG/EV + DOX). N.S., not significant. c The miR-181c expression levels were evaluated under the same experimental conditions as in (b) using the Taqman assay. *p-value < 0.05 and **p-value < 0.01