Fig. 2

Mmu-miR-125b inhibits NO production and iNOS mRNA expression in LPS-activated RAW264.7 cells and peritoneal macrophages. a The relative expression of mmu-miR-125b was determined in RAW264.7 cells infected with the pLL3.7-mmu-miR-125b lentivirus and sorted by FACS for GFP expression. b RAW264.7 cells overexpressing mmu-miR-125b (RAW264.7-miR-125b) and control cells (RAW264.7-pLL3.7, pLL3.7) were stimulated with 1 μg/ml LPS for 6 h. The supernatants were collected to measure NO using a nitrate/nitrite assay kit and normalized to the expression of total proteins. c iNOS mRNA levels were measured by qPCR and normalized to the expression of β-actin. d Peritoneal macrophages were transfected with mmu-miR-125b mimics or controls at a final concentration of 40 nM for 24 h. The cells were stimulated with 100 ng/ml LPS for 6 h, iNOS mRNA levels were measured by qPCR and normalized to the expression of β-actin. The data are presented as the mean ± SD (n = 3) of three independent experiments. e The supernatants were collected to measure NO using a nitrate/nitrite assay kit and normalized to the expression of total proteins. **p < 0.01; *p < 0.5