Fig. 5

Soluble E-selectin enhances homing of 4T1 cells to the lung in mice: a Effect of sE-selectin on infiltration of 4 T1 cells into the lung. Luciferase-expressing 4 T1 murine breast cancer cells were pre-incubated with sE-selectin (100 nM) and, following washing with PBS to eliminate sE-selectin carryover, were intravenously injected into female Balb/C or E-selectin knockout mice. Seven days later, lungs were harvested and luciferase mRNA was analyzed by qRT-PCR and normalized by GAPDH. The data represent Mean ± SD. Statistical significance was determined by Student’s t-test. b sE-selectin-mediates adhesion is ICAM-1 dependent. Parental-HMVECs were pre-incubated with monoclonal antibody (1 μg in 100 μl) against ICAM-1 or VCAM-1 or the same amount of normal IgG for 1 h at 37 °C prior to the adhesion assay. After a brief wash, MDA-MB-231 BCs that were treated with sE-selectin for 30 min were infused into parallel flow chambers. The data represent Mean ± S.D. **p < 0.01; ***p < 0.001 vs. -/-, Student’s t test. Data were summarized as % of control (-/-) from an experiment conducted in triplicate, and repeated twice