Table 4 Operational analysis of two-step KRAS mutation testing of 120 CRC samples

HRM

n

%

n

%

n

%

 Number of samples

120

100.0

20

100.0 (16.7)

120

100.0

 Analysis passed

89

74.2

18

90.0

105

87.5

  WT (total)

60

67.4

12

66.7

71

67.6

  WT (skewed HRM curve)

3

5.0

2

16.7

4

5.6

  Mutant (total)

29

32.6

6

33.3

34

32.4

  Mutant (skewed HRM curve)

1

3.4

1

16.7

1

2.9

 Analysis failed

31

25.8

2

10.0

Pyrosequencing

n

%

n

%

n

%

 Number of samples

19

100.0 (15.8)

3

100.0 (2.5)

19

100.0

 Analysis passed

18

94.7

2

66.7

18

94.7

  WT

12

66.7

0

0.0

13

72.2

  Potential low level mutation

2

11.1

1

50.0

0

0.0

  Mutant

4

22.2

1

50.0

5

27.8

 Analysis failed

1

5.3

1

33.3

1

Combined HRM + Pyrosequencing

n

%

 Number of samples

120

100.0

 Number of HRM runs

140

116.7

 Number of pyrosequencing runs

22

18.3

 Analysis passed

119

99.2

 WT

81

68.1

 Mutant

38

31.9

 Analysis failed

1

0.8