Fig. 2

CHEK2 represses the expression/secretion of SDF-1 and IL-6. a Whole cell lysates were prepared from the indicated cells and were used for immunoblotting analysis. The numbers below the bands indicate the corresponding expression levels after loading correction against GAPDH. b Total RNA was extracted from the indicated cells and used to assess the mRNA levels of SDF-1 and IL-1 using qRT-PCR and specific primers. c Conditioned media from the indicated cells were collected after 24 h and the levels of the indicated proteins were determined by ELISA, and were presented in the respective histograms. Error bars represent means ± S.D, *, p value < 0.0001