Fig. 1

Chemotherapy restores deficient immune response in breast cancer patient. a. Representative contour plots of SSC-A vs CD3 in Lymphocyte cells (by SSC-A vs FSC-A) after 72 h of polyclonal stimulation with anti-CD3/CD28/CD2 micro beads, 2d-aDCs or a combination of micro beads in addition to 2d-aDCs in healthy donors (n = 12), and breast cancer patients (n = 8) (before and after anti-TTx), numbers inside correspond to MFI of CD3 in lymphocyte gate (SSC-A vs FSC-A). b. Contour plots of CD69 percentage expression in T cells (CD3+) as in panel A, numbers correspond to the percentage of CD69 expressing cells in CD3+ T cells. c. Delta (from left to right) of IFN-γ, IL-8, IL-1β, and IL-6 cytokines relative to unstimulated control in healthy donors (Dashed bars) (n = 6) and MCC-002 breast cancer patient (before and after anti-TTx white and black bars respectively), bars show SEM. X: not detected. Results of experiments presented in panel c are representative of three performed