Fig. 1

Re-evaluation of the discordant cases: a 303: Using the Ventana IHC protocol the staining of the tissue core was annotated as positive (strong staining in 1 % of the tumors cells). The corresponding whole section did not show any protein expression. The Dako protocol was negative on both the TMA and the whole section. The FISH analysis was also negative. b L694: Using the Ventana IHC protocol the staining of the tissue core was annotated as positive (strong staining in 2–10 % of tumors cells). The corresponding whole section did not show any protein expression. The Dako protocol resulted in an opposite assessment with a negative result on the TMA and a positive result on the whole section. The FISH analysis was negative for this case. c 223: This case was annotated as positive with the Dako protocol on the TMA (moderate staining in 41–50 % of tumor cells) but was scored negative when the whole section was evaluated. This case was defined as positive both with Ventana IHC assay and the FISH assay. d L826: This case was annotated as positive with the in house Dako protocol on the TMA (strong staining in 41–50 % of tumor cells) but was scored negative when the whole section was evaluated. The Ventana IHC assay and the FISH analysis were negative on the TMA as well as on the whole sections. The scale bar in all images represents 100 μm