Fig. 3

uPAR directly interacts with uPA and IGF1R. a Western blot analyses of co-immunoprecipitation replicates (co-IP 1–3) of uPAR with uPA or with IGF1R (c), the lysate (L) of MDA-MB-231 cells was used as positive control and a non-targeting isotype antibody was used as negative control (NK) b PLA and quantification of protein-protein complexes of uPAR with uPA or with IGF1R (d) on cell block sections from mock and uPAR-depleted cells (uPAR-RNAi)