Fig. 2

AE1 and p16 affected AE2 localization and expression in GC cells. a AE1 or p16 knockdown increased AE2 expression on the surface of SGC7901 cells. Nuclei were stained with DAPI (blue). b AE1 and AE2 expression in the membrane and cytoplasmic fractions of SGC7901 cells was detected by western blot. Cellular AE1 (up panel) and p16 (bottom panel) expression was suppressed by transfection with AE1- or p16-targeted siRNA or shRNA. c, d Silencing of AE1 (c) or p16 (d) increased AE2 expression in SGC7901 cells as verified by western blot. The ratio of AE2 protein abundance to that of β-actin was normalized to a value of 1.0 for SGC7901, *p < 0.05, compared with siRNA-NC/pSIREN-NC. e, f AE1 (e) or p16 (f) overexpression reduced AE2 abundance in MKN28 cells. The ratio of AE2 protein abundance to that of β-actin was normalized to a value of 1.0 for MKN28, *p < 0.05, compared with empty vectors. The values are expressed as mean ± SD of three different experiments performed in triplicate