Fig. 1
From: Mechanism of action of trabectedin in desmoplastic small round cell tumor cells
JN-DSRCT-1 cell line resembles DSRCT disease characteristics. a 10X image of JN-DSRCT-1 cells. b EWS-WT1 fusion transcripts were detected in JN-DSRCT-1 cells by FISH. Chromosome preparation from JN-DSRCT-1 cells hybridized with WT1 break-apart BAC probes: Spectrum Orange labeled RP1- 259 N9 (centromeric WT1 5’ end) and Spectrum Green labeled RP11-299P16 (telomeric WT1 3’ end). A fusion signal (corresponding to a non-translocated WT1 allele) with two green signals (derivative chromosome 22) and an orange signal (derivative chromosome 11) are present in the metaphase andin the interphase nucleus. The FISH pattern is coherent with EWS break-apart (not shown). c Trabectedin chemical structure and Clonogenic assay on JN-DSRCT-1 cells. The IC50 was calculated by PRISM GraphPad. d Cell cycle analysis after 1 h of treatment with trabectedin; the data were analyzed 24, 48 and 72 h after drug wash-out