Fig. 2

Knockdown of PANDAR inhibited ccRCC cell proliferation and invasion in vitro. a. PANDAR expression levels in 7860 and Caki cells transfected with si-NC or si-PANDAR were detected by qRT-PCR. b. The cell proliferation of 7860 and Caki cells transfected with si-NC or si-PANDAR was measured by CCK-8. c. Colony formation assays were performed to detect the proliferation of 7860 and Caki cells that were transfected with si-NC or si-PANDAR for 15 days. d. Transwell assays were performed to investigate the invasive ability of 7860 and Caki cells that were transfected with si-NC or si-PANDAR. The number inside the bars represent the relative ratio of invaded cells (normalized to the control). The lysates of 7860 and Caki cells were detected by Western blotting assays. Data represent mean ± S.D., (n = 3) *P < 0.05; **P < 0.01