Fig. 1
From: Fluorescent CXCR4 targeting peptide as alternative for antibody staining in Ewing sarcoma
Semi-quantitative detection of MSAP-Ac-TZ14011 in EWS cell lines correlated significantly with CXCR4 RNA expression levels. a Cells of the EWS cell lines A673, IARC-EW7, L1062, 6647 and TC32 were harvested and stained without (upper graphs) or with (lower graphs) MSAP-Ac-TZ14011. Fluorescence was detected at 710–40 nm. The calculated percentage of positive cells is indicated in each panel. b The baseline corrected geometric mean cytometry fluorescence intensities (GFI) detected after MSAP-Ac-TZ14011 staining of the in (a) described EWS cell lines were correlated to the previous determined CXCR4 RNA expression levels (x-axis). Linear regression analysis demonstrated a significant correlation between the by MSAP-Ac-TZ14011 detected CXCR4 levels and RNA expression levels (P-value and 95% certainty borders are displaced). Both figures are representatives (n = 3)