Fig. 3

PAK1 knockout increased B- and T-lymphocytes in the spleen of APC^∆14/+ mice. Fresh spleens were dissected out from 10-week old PAK1 WT APC^+/+ mice (n = 13), PAK1 KO APC^+/+ mice (n = 10), PAK1 WT APC^∆14/+ mice (n = 18) and PAK1 KO APC^∆14/+ mice (n = 16). B220⁺, CD3⁺, CD4⁺ and CD8⁺ lymphocytes were analysed by flow cytometry. The percentage of positive cells was calculated using the Weasel computer program (Cytometry laboratory, WEHI, Parkville). Samples from at least 10 mice of each genotype were analysed **, p < 0.01 compared to PAK1 WT APC^+/+ mice. #, p < 0.05, ##, p < 0.01 compared to PAK1 WT APC^∆14/+ mice