Fig. 4
From: Inhibition of PI3K-AKT-mTOR pathway sensitizes endometrial cancer cell lines to PARP inhibitors
Reduced RAD51 foci formation in PTEN mutated endometrial cancer cells. Baseline RAD51 protein expression in the endometrial cancer cells (a and b). Cells were treated with 500 nM of Doxorubicin for 1 h, allowed to recover for 6 h and then fixed for immunofluorescence. Immunofluorescence staining images of RAD51 foci in nuclei of endometrial cancer cells with respect to the PTEN protein expression are shown at 100X magnification (c). Quantitative representation of the percentage of cells with positive RAD51 foci is shown in fig (d). PI3K inhibition decreases doxorubicin-induced RAD51 foci formation in these cells (e and f). Cells with >5 foci/nucleus were considered positive. Results represent the average of three independent experiments. *p < 0.05