Fig. 2
From: Therapy response testing of breast cancer in a 3D high-throughput perfused microfluidic platform
Culture optimization in the microtiter microfluidic platform. Up to 96 multiple conditions such as seeding density, ECM composition, cell types and perfusion can be investigated concurrently. Breast cancer cell line MDA-MB-453 was seeded in three different ECM compositions at two different densities and maintained for 6 days before assessment with a live/dead assay (Calcein AM - green/NucBlue® (Hoechst) - blue/NucRed® (propidium iodide) - Red). Scale bar = 400 μm. a Epifluorescence microscopy images showing different morphologies and viabilities of MDA-MB-453 in Matrigel®, BME2rgf and collagen I under static and perfusion conditions at a seeding density of 10*106 cells/mL. b Graphs quantifying the effect of ECM (Matrigel® vs BME2rgf vs collagen I), seeding density (10*106 cells/mL, black, vs 20*106 cells/mL, grey), and static vs perfusion culture on the viability (represented as % of total cells) of MDA-MB-453 cells. Total cell number was determined by nuclear count (Hoechst staining). Total number of dead cells was determined by positive propidium iodide staining. Viable cells was set at total cell number minus dead cell count