Fig. 1

Surface marker expression in Rb Y79 cell line using flow cytometry. Gating of single cells and voltage channel setting- (a) FSC vs SSC plot, (b) and (c) Doublet discrimination plots (d) Unstained control. Analysis of various surface markers with representative distribution of various sub-populations within the cell line (e) CD133-APC (f) CD44-PE (g) CD90-FITC, (h) CXCR4-APC (i) ABCB1-FITC. j and (k) Purity of sorted CD133^lo and CD133^hi cells was recorded as >90%. Cell cycle analysis of the total Y79 cells, CD133^lo and CD133^hi subsets highlighting the G0/G1 status of CD133^lo cells (l) Cell cycle distribution of total Y79 cells (m) CD133^lo cells predominantly observed in the G0/G1 phase (83.3 ± 4.1%) (n) CD133^hi cells mainly observed in the proliferating S/G2/M phase (81.1 ± 4.1%)