Table 1 Primer sequences for the genes used in semi-quantitative PCR
From: In vitro characterization of CD133lo cancer stem cells in Retinoblastoma Y79 cell line
S No. | Gene | Forward primer | Reverse primer |
1. | ACTB | atgcagaaggagatcactgc | tcatagtccgcctagaagca |
2. | CD133 | cctctggtggggtatttctt | aggtgctgttcatgttctcc |
3. | BMI1 | gcttcaagatggccgcttg | ttctcgttgttcgatgcatttc |
4. | NANOG | caaccagacccagaacatcc | ttccaaagcagcctccaag |
5. | OCT4 | atgcattcaaactgaggtgcctgc | ccaccctttgtgttcccaattcct |
6. | PROX1 | caagttgtggacactgtggt | gcagactggtcagaggagtt |
7. | MACC1 | cggtcaggaagaattgcac | ttaccacgaagggtgaaagc |
8. | SNAI2 | tgtgacaaggaatatgtgagcc | tgagccctcagatttgacctg |
9. | ABCG2 | ggaactcagtttatccgtgg | cgaggctgatgaatggagaag |