Fig. 5

miR-486-5p directly targets PTEN. a Sequence alignment of miR-486-5p and its conserved target site in the PTEN 3′-UTR. b Luciferase activity was measured in 293 T cells with a dual luciferase reporter assay. The cells were co-transfected with a plasmid expressing miR-486-5p mimic or control miRNA and a vector expressing PTEN 3′-UTR or empty vector. Firefly luciferase activity was normalized to Renilla luciferase activity, and the value for the empty vector was used as the control. **P < 0.01, compared with all other groups