Fig. 4

miR-199a/b-3p suppresses gastric cancer cell proliferation via down-regulating PAK4 expression in vivo. a MGC-803 cells transfected with negative control (NC) or 50 nM miR-199a/b-3p mimics respectively, were implanted into the axillary fossae of each mouse, after 2 weeks, xenograft tumors were removed intactly. Top, representative tumor images; the bar represents 1 cm. Bottom, quantitative analysis of tumor volume. b Total RNA and protein were isolated from xenograft tumors, and expression of PAK4 mRNA (bottom) and protein (top) was detected using real-time PCR and western blot, respectively

Data are shown as the mean ± SD (n = 8). n.s., not significant. *P < 0.05; **P < 0.01.