Fig. 5From: TIPE3 differentially modulates proliferation and migration of human non-small-cell lung cancer cells via distinct subcellular locationOverexpression of exogenous TIPE3 promotes the proliferation and migration of lung cancer cells. a, b TIPE3 with C-terminal flag was stably overexpressed in A549 cells by lentivirus transfection. GFP was detected by immunofluorescence (a), and flag was detected by western-blot (b). c The growth curves of A549 cells overexpressing TIPE3 were determined by CCK8 assay (n = 6 per timepoint). d, e The migration of A549 cells overexpressing TIPE3 was determined. Representative (d) and statistic (e) data (n = 5 per group) are shown. f, g The migration of A549 cells overexpressing TIPE3 was determined in the absence or presence of siTIPE3. Representative (f) and statistic (g) data are shown (n = 5 per group). Bars represent mean ± s.e.m. *P < 0.05, ***P < 0.001Back to article page