Fig. 5
From: Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas
Association between TIMP-1 and CD63. a-c Representative haematoxylin-eosin, CD63, and TIMP-1 staining of a diffuse astrocytoma (DA). d-f Representative haematoxylin-eosin, CD63, and TIMP-1 staining of an anaplastic astrocytoma (AA). g-i Representative haematoxylin-eosin, CD63, and TIMP-1 staining of a glioblastoma (GBM). j Using the TCGA dataset, low TIMP-1 levels were significantly associated with improved survival in glioblastoma patients when dichotomized at the 25th percentile. k Proximity ligation assay performed on glioblastoma biopsies visualizing TIMP-1 and CD63 proteins in close molecular proximity. A red dot appears when proteins are located sufficiently close together. The nuclei were visualized with DAPI (blue color). l Immunoblotting using an anti-TIMP-1 antibody showing TIMP-1 and CD63 binding in U87MG cell line-derived spheroids. Cell lysates were immunoprecipitated with an anti-CD63 (lane 2) and the corresponding IgG isotype as control (lane 1). Scale bar 50 μm. Asterisks indicate blood vessels