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Fig. 5 | BMC Cancer

Fig. 5

From: Knockdown delta-5-desaturase in breast cancer cells that overexpress COX-2 results in inhibition of growth, migration and invasion via a dihomo-γ-linolenic acid peroxidation dependent mechanism

Fig. 5

D5D-KD and DGLA supplementation enhance the efficacy of 5-FU on growth of 4 T1 cells. a Colony formation assay of D5D-KD 4 T1 cells or control siRNA transfected cells at 10 days with treatment of 5-FU (20 μM) or 5-FU + DGLA (100 μM) for 48 h. Note, D5D-KD and NC-si control cells without DGLA and 5-FU treatment (shown in Fig. 3) were used to calculate survival fractions; and b Cell apoptosis was examined via flow cytometry after D5D-KD 4 T1 cells were treated with DGLA (100 μM), 5-FU (20 μM), or 5-FU + DGLA for 48 h, followed by Annexin V-FITC/PI double staining Data represent as mean ± standard deviation (*: significant difference vs. control with p < 0.05; and #: significant difference vs. 5-FU group with p < 0.05 from n ≥ 3)

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BMC Cancer

ISSN: 1471-2407

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