Fig. 3

miR-451a inhibition increases tumor cell proliferation and clonogenic survival. HCT-116 were transfected with a miR-451a inhibitor (anti-miR-451a) or a control anti-miR. Proliferation was analyzed 48 h after radiation in a 2D and b 3D cultures with the indicated doses. Bars depict mean ± s.e.m. of triplicate wells. ** indicates P < 0.01 on a two-tailed Student’s T-test. c 12–14 days after plating, cells were fixed and stained with crystal violet and colonies were counted. Surviving fraction was calculated based on the colony numbers normalized to the plating efficiency. Mean of triplicate wells is plotted. * indicates P < 0.05 and ** indicates P < 0.01on a two-tailed Student’s T-test