Fig. 2
ANGPTL4 is induced by HIF-1α and HIF-2α in osteosarcoma cells (a) Expression of HIF-1α and full-length ANGPTL4 was assessed in 7 osteosarcoma cell lines exposed to hypoxic conditions (0.5% O2, 18 h). b, c Induction of ANGPTL4 in MG-63 and OS18 cells was observed after 18 h exposure to dimethyl oxalyl glycine (DMOG, 1 mM) by both (b) western blot and (c) ELISA. d Western blot showing complete knockdown of HIF-1α and HIF-2α protein in hypoxic MG-63 cells using isoform-specific HIF-1α and HIF-2α siRNA. e Effect of HIF-1α and HIF-2α siRNA on ANGPTL4 secretion in normoxic and hypoxic MG-63 . *p < 0.05; **p < 0.005; ***p < 0.001 versus the corresponding normoxic or hypoxic control