Fig. 3

Oncolytic activity exhibited by rFMW/GFP against ATC cells in both 2D and 3D cultures. a THJ-16 T and THJ-29 T cells were vehicle-infected or infected with varying MOI of rFMW/GFP (0.01, 0.1, 1 and 10) for 24, 48, 72 h respectively. Cell growth inhibition was determined using the MTT assay. b THJ-16 T and THJ-29 T cells were infected (similar to A above) with the same as in A for 6, 12 and 24 h. Expression levels of total and cleaved caspase-3 and PARP were analyzed by IB. β-actin was used as a control for equal loading. c 3D cultures of THJ-16 T and THJ-29 T cells were infected (similar to A above) for 48 h and examined for spheroid formation. Results are expressed as number of spheroids/1000 cells ± SEM, ***p < 0.001. (Scale bar = 100 μm). Data are representative of the mean ± SEM (***p < 0.001)