Fig. 1

NETs promote hypercoagulability through platelet aggregation. Tumor bearing mice have elevated platelet aggregation compared with sham controls (a, AUC 40.2 ± 5.5 vs. 25.8 ± 1.5, n = 5). Treatment of human (b) and murine (c) blood with NET supernatant led to a dose dependent increase in platelet aggregation compared with treatment with media control. Tumor bearing PAD4 KO mice had decreased platelet aggregation compared to WT (AUC 8.4 ± 2.4 vs. 3.7 ± 1.7, n = 7) with no difference in sham controls (d). *p < 0.05