Fig. 4

Docetaxel induces VEGFR3-dependent morphological changes of tumor-draining lymphatics in vivo. Representative images of 4T1 tumor-bearing fat pad tissue sections from mice treated as outlined in Fig. 1. Sections were immunostained for lymphatic marker podoplanin (black) and nuclei (green). Left panel (scale bar = 4 mm) shows whole tissue sections and middle panel (scale bar = 500 μm) shows peritumoral stroma. Red dashed lines show border of inguinal lymph node, blue dashed line shows border of tumor, and black arrows indicate lymphatic vessels. Right panel (scale bar = 125 μm) shows representative images of morphology of individual lymphatic vessels