Table 2 List of top ten (10) downregulated genes in gnidilatidin-treated B16F10 murine melanoma cells as determined by DNA microarray (Expression ratio of gnidilatidin vs Control)^a

Id2, inhibitor of DNA binding 2

Promotes tumor cell migration and invasion; The protein ID2 belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loophelix (HLH) domain but not a basic domain.

Sytl2, synaptogaminlike 2

The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTPbound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27Adependent vesicle trafficking and controls melanosome distribution in the cell periphery.

Tbca, tubulin cofactor a

Tubulin-folding protein; involved in the early step of the tubulin folding pathway.; The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates.

Sorbs1, Sorbin and SH3 domaincontaining 1

This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance.

Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).

Zfp85-rs1, Zinc finger protein 85, related sequence 1

Regulation of transcription, DNA-templated; nucleic acid binding; metal ion binding; transcription corepressor activity

Esco2, Establishment Of Sister Chromatid Cohesion NAcetyltransferase 2establishment of cohesion 1 homolog 2

Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA eplication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during the S phase. Acetylates the cohesin component SMC3.

Mtss1, metastasis suppressor 1

May be related to cancer progression or tumor metastasis in a variety of organ sites, most likely through an interaction with the actin cytoskeleton.

Zfp60, zinc finger protein 60

Negative regulator of cartilage differentiation

Psg28, pregnancyspecific glycoprotein 28

Relevant for cell adhesion and for positive regulation of endocytosis, phagocytosis, and inflammatory response

Brca1, breast cancer 1

E3 ubiquitin-protein ligase that specifically mediates the formation of Lys-6-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. Required for appropriate cell cycle arrests after ionizing irradiation in both the Sphase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage.