Fig. 5

MEK inhibitor suppressed spheroid formation in ID8-KRAS cells. ID8-KRAS-GFP cells (2 × 10⁵) were not treated (0 nM) or treated with trametinib (1, 10, 100 nM) for 48 h in 2D or 3D culture. a Representative microscopic image of cancer cells under 2D and 3D conditions at 100× magnification. b-c After the treatment, cells were exposed to EdU (10 μM) for 2 h and fixed. EdU was labeled with Alexa Fluor 647. EdU uptake was analyzed (b) and the ratio of EdU-positive to EdU-negative cells was measured by flow cytometry (c). Experiments were repeated at least thrice. The values shown represent mean ± SEM (* p < 0.05)