Fig. 3

Expression of HOXB4/miR-10a according to epigenetic modifiers mutational status. a NGS sequencing of TET2, ASXL1, EZH2, DNMT3A and IDH1/2 was performed on genomic DNA from the cohort of hematological malignancies described in Additional file 2: Table S2. Relative expression of HOXB4 and miR-10a were measured by qRT-PCR. b CD34⁺ primary hematopoietic cells purified from cord blood were transduced with a lentivirus allowing the expression of WT or R882H mutated DNMT3A and grown in complete medium containing SCF, TPO and Flt3-L. After 3 days of selection in the presence of puromycin (1 μg/mL), the expression of miR-10a was assessed by qRT-PCR. Results are expressed as the expression of miR-10a normalized on RNU6–1 expression and compared to the WT condition (n = 3). *, p < 0.05; **, p < 0.01; NS, non-significant