Fig. 1

SiRNA targeting BKCa successfully suppressed the expression of BKCa and inhibited the influence of the biological effects of endometrial adenocarcinoma cell line Ishikawa. a, b The siRNA targeting BKCa effectively down-regulated the mRNA and protein level of BKCa expression at time point of 48 h post-transfection. c Knockdown of BKCa significantly inhibited cell proliferation at 48 h and 72 h after transfection of siRNA (p = 0.035, p = 0.005). d At 48 h after transfection, apoptosis assay was performed to determine the apoptotic rate of the two groups of Ishikawa cells. Knockdown of BKCa increased the apoptosis rate from 8.57 ± 1.07% to 30.97 ± 2.93% (p = 2.4E^− 4). e FACS was used to analyze the alteration of cell cycle distribution induced by knockdown of BKCa expression. SiRNA targeting BKCa induced blocking of cell cycle progression and resulted in accumulation of G1 stage (p = 2.8E^− 4). f, g Representative images of migration and invaded cells following knockdown of BKCa expression in Ishikiwa cells were showed by crystal violet staining. SiRNA targeting BKCa reduced significantly the numbers of migration (p = 3.18E^− 4) and invaded (p = 0.001) cells. Data (Mean ± SD, n = 3 independent experiments)