Table 1 List of the used antibodies, antigen retrieval (AR), dilutions and Opal™ dyes
From: Expression of the immune checkpoint receptor TIGIT in Hodgkin’s lymphoma
Bright field | Fluorescence | ||||||
|---|---|---|---|---|---|---|---|
Antibody | Target | AR | Dilution | AR | Dilution | Order1 | Dye |
DAKO #IR503 | CD3 | pH 9 | 1:1 | pH 9 | 1:1 | 1st | Opal 520 |
DAKO #IR649 | CD4 | pH 9 | 1:1 | pH 9 | 1:1 | 1st | Opal 520 |
DAKO #IR623 | CD8 | pH 9 | 1:1 | pH 9 | 1:1 | 1sta | Opal 520b |
BioLegend #320102 | FOXP3 | pH 9 | 1:50 | pH 9 | 1:50 | 1st | Opal 520 |
DAKO #IR604 | CD20 | pH 9 | 1:1 | pH 9 | 1:10 | 1sta | Opal 520b |
Dianova #DIA-TG1 | TIGIT | pH 7.8 | 1:70 | pH 9 | 1:150 | 2nd | Opal 570 |
Abcam #ab52587 | PD-1 | pH 6 | 1:50 | pH 6 | 1:50 | 3rd | Opal 690 |