Table 2 List of human primer and probe sequences used for qRT-PCR
Gene Name | RefSeq No. | Exon Location | Primer | Probe |
|---|---|---|---|---|
MEF2A | NM_005587 | 13–14 | 5’-GGTTCGGACTTGATGCTGAT-3’ | 5′−/56-FAM/AACCCTGAG/ZEN/ ATAACTGCCCTCCAG/3IABkFQ/− 3’ |
5’-CACCACCTAGGACAAGCAG-3’ | ||||
MEF2B | NM_001145785 | 1–2 | 5’-GATGCGGGAGATCTGGATT-3’ | 5′−/56-FAM/CATCGTCCC/ZEN/ AGGCTGAGTGGAAT/3IABkFQ/−3’ |
5’-CCCCTGATCTTCGTGCAG-3’ | ||||
MEF2C | NM_002397 | 3–4 | 5’-TGTTGGTGCTGTTGAAGATGA-3’ | 5′−/56-FAM/TGCTGTGTG/ZEN/ ACTGTGAGATTGCGC/3IABkFQ/−3’ |
5’-AGATTACGAGGATTATGGATGAACG-3’ | ||||
MEF2D | NM_001271629 | 11–12 | 5’-CTGCACTGGTCAACTGGTAA-3’ | 5′−/56-FAM/CTCCCCTTC/ZEN/ TCTTCCATGCCCAC/3IABkFQ/−3’ |
5’-CAGTCTACTCATTCGCTCACC-3’ | ||||
GAPDH | NM_002046 | 2–3 | 5’-TGTAGTTGAGGTCAATGAAGGG-3’ | 5′−/56-FAM/AAGGTCGGA/ZEN/ GTCAACGGATTTGGTC/3IABkFQ/−3’ |
5’-ACATCGCTCAGACACCATG-3’ | ||||
TBP | NM_003194 | 5–6 | 5’-CAAGAACTTAGCTGGAAAACCC-3’ | 5′−/56-FAM/CACAGGAGC/ZEN/ CAAGAGTGAAGAACAGT/3IABkFQ/−3’ |
5’-GATAAGAGCCACGAACCAC-3’ |