Fig. 3

SEMA5A knockdown enhances tumor size and metastatic potential. a-c Graph showing increase in the average weight of the mice in grams (p = 0.034), (a) the average weight of primary tumor in grams (p = 0.0159) (b) and number of metastases per liver (p = 0.0079) (c) in mice orthotopically injected with T3M-4-shSEMA5A cells in comparison with mice injected with T3M-4-Control cells. The error bars in the graphs presented in this section represent ± Standard Deviation and significance of the data is calculated using Mann-Whitney U-Test. d Representative images showing SEMA5A and PCNA expression and H&E staining of orthotopic pancreatic tumors from T3M-4 shSEMA5A and Control cells. Scale bar represents 10 μm in length. Images are taken using a Nikon Eclipse E800 microscope. e Bar graph showing a higher number of proliferating tumor cells stained with PCNA (p = 0.023) in athymic mice orthotopically injected with T3M-4-shSEMA5A in comparison with those injected with Control cells. The error bars in the graphs represent ± SEM and * indicates statistical p-value less than 0.05 using Student t-test