Fig. 1

Dietary intake of genistein delays initiation and suppresses development of HCC. a Schedule of the dietary intake of genistein. HCC bearing mice were fed genistein with combined chow for 5 months from the age of 40 weeks to 62 weeks. b Representative images showing HCC development in 62-week-old livers of female control and genistein groups that were DEN-treated mice. Hematoxylin and eosin (H&E) staining (Scale bar, 20 μm) of liver is presented. Pyknotic nuclei in tumor cell is stained with dark hematoxylin. c Tumor burden (mm²) quantified by Image J, incidence, numbers, and liver weight per body weight in 62-week-old livers of female control and genistein groups. Values represent means ± SEM. *, p < 0.05. Numbers of mice used in experiment is at least each 6 for control and genistein groups