Fig. 5

Principal component analysis (PCA) of promoter associated DNA methylation. Average methylation of 155,931 promoter associated CpGs on 115 ccRCC and 12 tumor free (TF) adjacent tissue samples are plotted, after centring average beta values, using the first two principal components. All samples are represented by a dot and subgroups are highlighted according to (a) TF-tissue samples, cluster a and cluster b; (b) TF-tissue samples, ccRCC without distant metastasis at diagnosis that did not progress (M0-PF), ccRCC without distant metastasis at diagnosis that did progress (M0-P) and ccRCC with distant metastasis at diagnosis (M1)